6 attempts is usually the max. every attempt you do, you drop
annealing temp by 2 degrees. so the protocol prediction says the
primer should use 56 degrees celcius. at the 6th attempt, you're
using 44 degrees, (which is VERY off the "optimum" already) and
anything lower than that there is no point even trying.
got 5 machines. but then have to let other people in the lab use
also mah... cannot just book for myself wad. plus each run takes 3
hours. a lot of waiting time in between loh.
