j3 7'aime

last SG · 2006-04-05T20:40:05+00:00

I no money buy... so I used those. Very limited, but bo pian. Now Microsoft gives out free domain names, but take very long to just to confirm you are using...

CoolMyth SG

Founded by your "kor". Laughing

http://death.sgforums.com/

starblue SG

i see you share my admiration for our resident biology expert. Mr. Green

alexkusu SG

starblue SG

really??

how about:

if you wan to prepare 500ml of agarose with EtBr 0.5microgram/ml. you have a stock solution of EtBr at 1mg/ml. how much EtBr u use?

a) 250microlitre
b) 500
c) 1000
d) 25
e) 100

CoolMyth SG

I just hope I can get free medical care in future nia. Laughing

LazerLordz SG

Peng san liao.

Okay, try this..

Choose one of the following for close analysis, paying attention to how dialogue advances this stage in the dramatic action, speech acts, stage business, role-playing, etc.

(a) Waiting for Godot, Act 2:

Vladimir. Poor Pozzo!
Estragon. I knew it was him.

[ ]

Estragon. I dont know.
Vladimir. You may be right.

Twisted Evil

alexkusu SG

tricky wor... normally i wud go for C..but let oxford answer that

starblue SG

now that's what i call foresight. Laughing

CoolMyth SG

Laughing

And hor, the free in me did some searches and how nice!

http://www.sgforums.com/?action=thread_display&thread_id=128829

It's coming to a year since you found your kor. Any comments?

starblue SG

wad u mean normally you will go for C? u dun calculate one meh??

haiz.... i suck at this math sht... T_T how i know biology also got to do maths?

LazerLordz SG

Laughing

starblue SG

comments ah??

1) i need a new kor

2) i need to open my eyes when i look for a new kor

Laughing

oxford mushroom SG

Wa...I come in only you test me huh?

Usually up to from half a kb to 15kb we use 0.8-1% gel but you can stretch it at both ends, depending on how long you want to wait and the fragment size you are targeting.

So below 500bp the usual thing is to use a polyacrylamide gel but if you want to stretch your agarose gel, you can use a higher concentration say, 2.5% to target smaller fragments up to 100bp. My colleague who was trained in the National Cancer Institute uses a whooping 10% gel to run overnight and she gets good results with small fragments.

On the other extreme, if you lower the concentration of your agarose gel, you can resolve larger fragments.

So although there are lots of caveats, I think the simple answer to your mcq would be 1%. However, one can argue that c and e are not completely wrong either...(c) might not be good enough for resolution whilst (e) will do if you let it run longer..

CoolMyth SG

Yo, dude. That's a damn fast reply. Shocked

You're operating on the NUS network? Laughing

oxford mushroom SG

you people post too quickly leow...old folks like me cannot catch up Sad

starblue SG

damn..... i changed my answer last min. -_-""

CoolMyth SG

Laughing

starblue SG

OM!! got another question, dun mind enlighten me a bit... actually got more questions one. but i cannot remember the details... today's test suck to the core. T_T

starblue SG

why?? u wanna be my kor?? Mr. Green

LazerLordz SG

Nope. Laughing

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