~The History of 30 Crapboxes etc ~

last SG · 2006-03-14T04:24:38+00:00

I was 54%. oh what a saint I am.

starblue SG

i have to design my own protocol for my experimental module. including how much of which reagent to add in wad sequence, at what incubation temperature for how long in what type of buffer of what concentration yada yada yada...

o.O how the F i will know?? -_-""

starblue SG

yes yes... so got any anti-depressants? hisoka boy needs some...

StarPuppy SG

aiya..with frens..anywhere is ok de.. Wink Wink Wink

hisoka SG

theres this thing called journals to help you

ndmmxiaomayi SG

Good evening. Very Happy

starblue SG

cant find any useful sht.... -_-""

can u help me?? Mr. Green i need protocol for using lipopolysaccharide to induce iNOS and/or COX-2 expression in mouse RAW264 cells.

hisoka SG

haha but i prefer to meet up with starblue but she so bad wants to cast me into forever depression Crying or Very sad

next time you got outing with whatever call me bah, see if ree or not hehe Mr. Green

starblue SG

hi there lil ant

StarPuppy SG

good evening mayi...

Embarassed

StarPuppy SG

Outing arh..what outing?

we got a Mushy Gang Outing (AA) coming very soon

thers the AA outing also

Very Happy

hisoka SG

clone the cells into several petri dishes. try injecting or just throwing the liposaccharide into the meduim Mr. Green

hisoka SG

whats the diff betw mushy gang(aa) outing and aa outing. anyway dun wnat lah dunno them one. and it hink mc dun like me Confused

starblue SG

-_-" i need the exact amount of wadever sht to add and the time for incubation and temperature. everything. it must look like a protocol for poeple to follow step by step.

hisoka SG

okz seriously go and find out what induces the 2 protein/whatever production. then from there try and think of a method lor. anyway i believe there should papers on it. go and use the web of science bah

edit: come to think about it. maybe if you use the liposaccharide to couple with the genes that induce produce the products you want(promoter and all) then go and inser the liposcaccharide-dna complex into the mouse cells.

LazerLordz SG

can you speak English my dear Blue? Shocked

ndmmxiaomayi SG

Hi starblue. Very Happy

StarPuppy SG

then have a SBH outing la..we not really that regulars but

wat the heck

simple_bear, me and mao la....and maybe if can..get the_bear to come down? Razz

Laughing

seow SG

Good night all!

Hello. Laughing

hisoka SG

thats english man. other than for whats speicifically the cell type and the product i can understand mah

LazerLordz SG

I'm not scientifically inclined.. Laughing

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