At your 7th point, you are supposed to mention that complementary sticky ends are
produced. YES.
At your 10th point, the enzyme mentioned is DNA ligase.
You don't have to mention it.
At your 12th point, your have to mention that you need to apply
temporal heat or shock to open up the pores of the bacteria's cell
membrane and hence allow the recombinant DNA to enter the bacterial
cell. You don't have to mention it.
And in any case, a fermenters' actual process no need to
know in depth just know that what are the conditions that the
fermenter needs to keep in order to maintain an optimum conditions
for the transgenic bacteria to grow and multiply quicky...i.e. ph,
oxygen, temperature, nutrient broth that contains
a carbon-containing energy source(glucose), nitrogen source(amino
acids) and also mineral salts. only thing you need to know
is bolded.
Bacterias are not used as vectors...rather the term vector is
used to refer to another form of DNA that can be transferred
from one organism to another, and in this case the vector is the
plasmid isolated from the bacteria. YES.
To add on, mention:
insulin produced in bacterial cell --> extract -->
purified --> for use
